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Table 6 Annotation results of new ATDs flanking sequences in heat-shocked plants from different HSP::TRANSPOSASE/ATDs double transgenic aspen lines without any phenotypic alterations ("blind" approach) grown in the greenhouse.

From: Ac/Ds-transposon activation tagging in poplar: a powerful tool for gene discovery

Transgenic line

Number of plants

Plant affiliation

Genomic insertion locus (scaffold:position)*

Score

E-value

Transcript (POPTR_)

Functional annotation

  

# 82

16:6,171,964

41.0

5.7e-2

  
  

# 147

8:7,052,973

248.3

1.1e-64

  

N82-5

5

# 210

9:11,936,320

114.9

1.1e-23

0009s15430

Carotiniod oxygenase

  

# 211

607:7,883

160.0

3.2e-37

0607s00230

No functional annotations for this locus

  

# 213

7:1,458,117

42.8

3.52e-3

0007s02460

Ubiquitin-like protein

N82-11

1

# 224

15:11,529,160

1103.1

0

  

N82-14

2

# 6

5:23,009,323

482.8

7.9e-135

  
  

# 76

16:13,632,466

452.1

9.8e-126

  
  

# 4

2:10,051,122

42.8

1.6e-2

  
  

# 73

12:3,471,598

488.2

2.1e-136

0012s04240

ABC-2 type transporter

  

# 28

19:12,716,418

223.1

1.3e-56

0019s11440

Protein of unknown function (DUF803)

  

# 110

4:21,317,871

1402.5

0

0004s22400

UDP-glucuronosyl and UDP-glucosyl transferase

N82-15

8

# 131

7:13,340,488

389.0

8.8e-107

0007s13230

Dolichyl pyrophosphate phosphatase and related acid phosphatases

  

# 137

2:10,620,751 and

729.8

0

  
   

2:10,621,198

268.2

1.8e-70

0002s14350

PHD-finger

  

# 138

20:865,030

91.5

2.4e-17

  
  

# 140

2:21,231,185

524.3

2.1e-147

0002s23690

Cysteine protease inhibitor activity

  

# 29

4:1,137,737

771.3

0

0004s01860

Cytochrome P450 CYP2 subfamily

  

# 85

10:16,307,759

787.6

0

0010s17860

Phosphoglycerate kinase [EC:2.7.2.3 ]

N92-1

6

# 151

17:141,236

194.2

2.8e-48

0017s00430

Serine/threonine protein kinase

  

# 155

8:6,405,007

403.4

5.0e-111

  
  

# 242

10:13,783,139

987.7

0

0010s14080

Diaphanous homolog 2

  

# 244

4:17,605,886

735.3

0

  

N92-4

1

# 93

10:13,739,252

336.7

6.,3e-91

  

N95-3

1

# 179

9:9,182,685

253.8

4.2e-66

0009s10820

Apoptosis-promoting RNA-binding protein TIA-1/TIAR

N95-6

2

# 176

1:42,827,910

740.7

0

  
  

# 190

1:2,422,398

459.3

8.0e-128

0001s03080

Type II intron maturase

  1. Genomic insertion locus (scaffold and position) with score, e-value and, if applicable, annotated transcript.
  2. In BLAST-analyses with more than one hit the one with lower e-value is shown.