Figure 5

Validation of age-related, astrocyte-specific expression changes in an independent dataset (A) and using an alternative astrocyte reference signal (B). A: Out of 50 genes with astrocyte-specific expression change selected from our first dataset, 30 were found to show expression changes in a second, independent dataset. The plot shows astrocyte-specific fold change in expression detected in the first dataset (x-axis) and second dataset (y-axis). Black and gray open circles code for the significance of the p-values for the expression change detected in the second dataset (black: p<0.05, 18 genes; gray: p>0.05, 12 genes). Even though the corresponding p-values were not all nominally significant, all 30 genes supported gene expression changes upon statistical model selection. B: Out of 50 genes with astrocyte-specific expression change selected from our first analysis, 42 were found to show expression changes using an alternative, independent reference signal. Even though the corresponding p-values were not all nominally significant, all 42 genes supported gene expression changes upon statistical model selection.