Similarity of type 3 secretion systems (T3SS). (A) Alignment of the whole genomes of P. sneebia, P. rettgeri, P. alcalifaciens, and P. burhodogranariea with the locations of each T3SS marked by blue boxes. (B) Alignment of T3SS-1 and some surrounding genes in P. sneebia, P. rettgeri, P. alcalifaciens, and Proteus mirabilis. Since this island falls within the inversion in P. sneebia’s genome, the region is displayed as the reverse-complement for ease of viewing. (C) Alignment of T3SS-2 and some surrounding genes in P. sneebia and P. burhodogranariea. (D) Alignment of T3SS-1 and T3SS-2 and some surrounding genes in P. sneebia. Figures were made using EasyFig. Lines connecting the sequence schematics indicate regions of similarity, with darker grey indicating greater similarity. Colored arrows indicate individual genes and their direction. The black boxes behind the genes indicate the approximate boundaries of the T3SS island based on gene annotation.