Figure 7

Chromatograms and mass spectra show the presence and absence of hassallidin variants in Anabaena sp. 90. A: Total ion chromatogram (TIC) of Anabaena sp. 90, originating in 1998, and extracted ion chromatograms (EICs) of Anabaena sp. 90, originating from 1998 and 2010, show the elution of ions with m/z 1200 – 1900, representing the mass range of hassallidin ions. Two eluting time ranges of the hassallidin variants are marked in the chromatograms. B-G: Average mass spectra from the first (B, D, F) and the second (C, E, G) time range of the chromatograms in which the hassallidin variants were eluting. The grey areas show the hassallidin ions in the spectra. B, C: Spectra from Anabaena sp. 90 originating from 1998. D, E: Spectra from Anabaena sp. 90 originating from 2010. F, G: Spectra from Anabaena sp. 90 anabaenopeptilide minus mutant originating from 2010