Salivary protein sequence comparisons among six anopheline species. (A) Phylogenetic relationships among six Anopheles species using the cytochrome oxidase subunit II (COII) protein sequences. Evolutionary analyses were conducted in MEGA5 . The Aedes aegypti sequence was taken as an outgroup. The tree is drawn to scale with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. (B) Clustal alignment. The numbers into brackets in the sequence titles indicate the NCBI accession number. The level of sequence identity is graphically represented above sequences alignment. (C) Average normalised BLAST scores ± standard deviations (numbers in bold into square brackets) and percentage identities (numbers in italic into brackets) between local alignments of secreted salivary proteins pertaining to sialomes from different Anopheles species. Pairwise protein-protein sequence comparisons were performed using “BLAST 2 Sequences”  (q.v. Additional file 1). This analysis of divergence among secreted salivary protein repertoires was carried out using all protein sequences from each Anopheles species matching at least one other salivary protein in another species at 40% identity (q.v. Additional file 2). The number of secreted salivary proteins used in each species is indicated into brackets.