Figure 2

Southern blot hybridization showing four distinct mitochondrial genome structures in Babesia microti (A) and Babesia rodhaini (B). (A) Genomic regions of probes Bm-1, Bm-2 and Bm-3 and restricted enzyme sites of Dra I and Eco 065I are shown in the lower map of the B. microti mt genome type-I (see Figure 1-A). Lanes 1, 4 and 7 for undigested DNA, lanes 2, 5 and 8 for Dra I-digested DNA, and lanes 3, 6 and 9 for Eco 0651I-digested DNA. (B) Genomic regions of probes Br-1, Br-2 and Br-3 and restriction enzyme sites of Hind III and Xho I are shown in the lower map of the B. rodhaini mt genome type-I (see Figure 1-B). Lanes 1, 4 and 7 for undigested DNA, lanes 2, 5 and 8 for DNA digested with Hind III, and lanes 3, 6 and 9 for DNA digested with Xho I (lanes 3, 6 and 9).