Skip to main content

Table 1 List of the 18 spots identified by LC-ESI-MS/MS whose concentration is modulated by leaf rust infection in barley leaves

From: Proteomic characterization of the Rph15 barley resistance gene-mediated defence responses to leaf rust

Spot ID

Accession number

Species

Protein description

EC

Abb .

M r a / pI a

M r b / pI b

Cov. (%) c

Photosynthesis and energy metabolism

     

2577

Q40073

Hordeum vulgare

Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic

 

RuACS (b)

40.2 / 5.3

46.1 / 5.6 e

43.5 e

2581

Q40073

Hordeum vulgare

Ribulose bisphosphate carboxylase/oxygenase activase A, chloroplastic

 

RuACS (c)

40.8 / 5.2

46.1 / 5.6 e

34.6 e

2760

P05698

Hordeum vulgare

Ribulose bisphosphate carboxylase large chain

4.1.1.39

RuLC

25.4 / 6.4

52.9 / 6.2 e

16.3 e

2776

P05698

Hordeum vulgare

Ribulose bisphosphate carboxylase large chain

4.1.1.39

RuLC

24.2 / 6.4

52.9 / 6.2 e

22.6 e

2866

P05698

Hordeum vulgare

Ribulose bisphosphate carboxylase large chain

4.1.1.39

RuLC

22.3 / 5.2

52.9 / 6.2 e

8.0 e

2952

P05698

Hordeum vulgare

Ribulose bisphosphate carboxylase large chain

4.1.1.39

RuLC

21.3 / 5.1

52.9 / 6.2 e

9.0 e

2983

P05698

Hordeum vulgare

Ribulose bisphosphate carboxylase large chain

4.1.1.39

RuLC

20.1 / 5.3

52.9 / 6.2 e

4.6 e

3004

XP _002465461

Sorghum bicolor

ATP synthase β chain d

 

ATPase β

19.4 / 4.1

22.8 / 5.3

6.1

3009

P05698

Hordeum vulgare

Ribulose bisphosphate carboxylase large chain

4.1.1.39

RuLC

19.2 / 5.2

52.9 / 6.2 e

4.2 e

3118

P05698

Hordeum vulgare

Ribulose bisphosphate carboxylase large chain

4.1.1.39

RuLC

19.7 / 5.0

52.9 / 6.2 e

6.9 e

3142

P05698

Hordeum vulgare

Ribulose bisphosphate carboxylase large chain

4.1.1.39

RuLC

20.4 / 4.7

52.9 / 6.2 e

4.4 e

3150

P05698

Hordeum vulgare

Ribulose bisphosphate carboxylase large chain

4.1.1.39

RuLC

20.7 / 4.8

52.9 / 6.2 e

4.8 e

Carbohydrate metabolism

     

2333

CAZ64535

Hordeum vulgare

Sucrose synthase

2.4.1.13

SuSy

88.2 /5.9

92.2 / 5.8

18.4

2962

CAC32847

Hordeum vulgare

Adenosine diphosphate glucose pyrophosphatase

 

AGPPase

21.0 / 5.1

19.5 / 5.7 e

17.4 e

Protein degradation

     

2449

XP _002454700

Sorghum bicolor

Leucine aminopeptidase 2, chloroplastic d

3.4.11.1

LAP 2

57.0 / 5.6

61.8 / 7.6

20.0

Defence responses

     

2886

CAA55345

Hordeum vulgare

chitinase

3.2.1.14

CHI

22.3 / 5.2

26.6 / 6.1

29.4

2956

BAD31057

Oryza sativa

Chitinase III-like protein

 

CHI III

21.2 / 5.3

18.9 / 6.5

12.7

Secondary metabolism

     

2613

CAA54616

Hordeum vulgare

Flavonoid 7-O-methyltransferase

2.1.1.6

F-OMT

39.2 / 5.4

42.3 / 5.4

29.5

  1. Statistical information about LC-ESI-MS/MS analysis are reported in Additional files1 and2.
  2. Abb.: abbreviation.
  3. a: experimental molecular weight and pI.
  4. b: theoretical molecular weight and pI.
  5. c: amino acid coverage (%).
  6. d: annotation obtained by BLAST-P alignment analysis against the Viridiplantae subset of the nr-database at NCBI.
  7. e: value referred to the mature form of the protein.