Figure 4

Sugar accumulation is required for ANE-mediated freezing tolerance in Arabidopsis: (A) sensitive phenotype of sfr4 mutant plants treated with (i) water control (ii) ANE (1.0 g L^-1) and (iii) LPC (1.0 g L^-1) to a temperature of −2.5°C for 24 h in Peat Pellet Freezing Tolerance Assay, and (B) extent of freezing-induced tissue damage in controls and treated sfr4 mutant plants as revealed by trypan blue staining; comparison of the area of tissue damage in the trypan blue stained leaves using the image processing and analysis software Image-j®. Data are means ± SE.