Table 3 ChIP-seq from primary cells isolated from human monozygotic twins
From: Limitations and possibilities of low cell number ChIP-seq
Twin Pair | Twin pair 1 | Twin pair 2 | Twin pair 3 | |||
|---|---|---|---|---|---|---|
CD4 + cells | ||||||
Cell no. / IP | 5 x 105 | 5 x 105 | 5 x 105 | 5 x 105 | 5 x 105 | 5 x 105 |
No. reads | 35 244 517 | 44 255 574 | 40 644 508 | 45 738 891 | 38 332 819 | 29 484 478 |
No. unique, nonduplicate reads | 3 978 339 | 6 524 094 | 3 316 312 | 3 340 926 | 3 130 804 | 1 167 824 |
No. Peaks called | 14 828 | 12 622 | 15 598 | 15 731 | 15 825 | 13 719 |
No. overlapping peaks (%) | 12 457 (82%) | 14 833 (94%) | 13 091 (83%) | |||
CD8 + cells | ||||||
Cell no. / IP | 4.3 x 105 | 3.7 x 105 | 4.7 x 105 | 5 x 105 | 3.8 x 105 | 4.2 x 105 |
No. reads | 45 245 361 | 35 681 254 | 38 455 042 | 35 257 788 | 34 505 357 | 41 729 689 |
No. unique, nonduplicate reads | 18 996 309 | 4 778 486 | 12 403 566 | 6 654 203 | 5 840 308 | 4 438 574 |
No. Peaks called | 17 704 | 18 720 | 20 728 | 18 145 | 18 743 | 20 899 |
No. overlapping peaks (%) | 14 578 (78%) | 18 828 (76%) | 15 312 (73%) | |||