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Figure 5 | BMC Genomics

Figure 5

From: Transcriptome characterization and gene expression of Epinephelus spp in endoplasmic reticulum stress-related pathway during betanodavirus infection in vitro

Figure 5

Sub-cellular localization of NNV capsid protein and BiP protein in NNV-infected GF-1 cells. Grouper fin cell line GF-1 was infected with NNV at MOI of 10. At 24 hr post-infection, the cells were fixed and co-stained with rabbit anti-NNV capsid and mouse anti-BiP, followed by Texas Red conjugated anti-rabbit IgG and Fluorscein conjugated mouse IgG. The Nucleus was visualized by counter-staining with 4', 6-diamidino-2-phenylindole (DAPI). The NNV capsid protein (A) is shown to co-localize with BiP protein (B) in distinct area within the cytoplasm as indicated by the merged image (C). Uninfected GF1 cell was indicated by white arrow.

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