Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library

Pan, Xian; Lei, Bingkun; Zhou, Nan; Feng, Biwei; Yao, Wei; Zhao, Xin; Yu, Yao; Lu, Hong

doi:10.1186/1471-2164-13-662

Table 1 List of genes whose deletions exhibited strong sensitivities to DNA damage reagents

From: Identification of novel genes involved in DNA damage response by screening a genome-wide Schizosaccharomyces pombe deletion library

Systematic ID	Gene name	Description of the gene products	DNA damaging agents	Flow cytometry phenotype^a	Reference^b
SPAC17A5.07c	ulp2 ⁺	SUMO deconjugating cysteine peptidase	HU, BLM, MMS, TBZ, UV	1C	[19]
SPAC1952.07	rad1 ⁺	checkpoint clamp complex protein	HU, BLM, MMS, CPT, UV	1C	[12, 13, 20]
SPAC23C11.15	pst2 ⁺	Clr6 histone deacetylase complex subunit	HU, BLM, MMS, TBZ, UV	2C	[21]
SPAC3C7.03c	rhp55 ⁺	RecA family ATPase	HU, BLM, MMS, TBZ, UV	2C	[12, 22]
SPBC1D7.04	mlo3 ⁺	RNA annealing factor	HU, BLM, MMS, CPT, UV	W4C	[13]
SPAC1952.05	gcn5 ⁺	SAGA complex histone acetyltransferase catalytic subunit	HU, MMS, CPT, TBZ	2C	[12, 13, 23]
SPAC227.07c	pab1 ⁺	protein phosphatase regulatory subunit	HU, MMS, TBZ, UV	S4C	[12, 13, 24]
SPAC4D7.10c	spt20 ⁺	histone acetyltransferase SAGA complex subunit	HU, CPT, TBZ, UV	S4C	[23]
SPAC6G9.10c	sen1 ⁺	ATP-dependent 5' to 3' DNA/RNA helicase	HU, BLM, TBZ, UV	2C	[25]
SPBC146.13c	myo1 ⁺	myosin type I	HU, BLM, TBZ, UV	2C	[12, 26]
SPBC2F12.11c	rep2 ⁺	transcriptional activator	HU, BLM, MMS, UV	1C	[13, 27]
SPBC3D6.04c	mad1 ⁺	mitotic spindle checkpoint protein	HU, BLM, TBZ, UV	NC^c	[13, 28]
SPBC342.05	crb2 ⁺	DNA repair protein RAD9 homolog	HU, BLM, MMS, UV	NC	[12, 13, 29]
SPBC409.15		rRNA processing protein	HU, MMS, TBZ, UV	S4C	[13]
SPCC1393.05	ers1 ⁺	RNA-silencing factor	HU, BLM, TBZ, UV	2C	[13]
SPCC306.04c	set1 ⁺	histone lysine methyltransferase	HU, BLM, MMS, TBZ	2C	[13, 30]
SPCC417.02	dad5 ⁺	DASH complex subunit	HU, BLM, TBZ, UV	NC	[12, 13, 31]
SPAC16E8.09	scd1 ⁺	Rho guanine nucleotide exchange factor	HU, BLM, TBZ	2C	[32]
SPAC4H3.05	srs2 ⁺	ATP-dependent DNA helicase	BLM, MMS, UV	1C	[12, 33]
SPAC6F6.01	cch1 ⁺	calcium channel	HU, MMS, UV	2C	[12, 13]
SPAC664.07c	rad9 ⁺	checkpoint clamp complex protein	HU, BLM, MMS	NC	[12, 13, 20]
SPBC11B10.10c	pht1 ⁺	histone H2A variant	BLM, MMS, UV	NC	[34]
SPBC13G1.08c	ash2 ⁺	Ash2-trithorax family protein, Set1 complex component	HU, BLM, TBZ	2C	[12, 30]
SPBC428.08c	clr4 ⁺	histone H3 methyltransferase	HU, MMS ,TBZ	1C	[12, 13, 35]
SPBC660.11	tcg1 ⁺	single-stranded telomeric binding protein	HU, BLM, TBZ	2C	[13]
SPBC800.05c	atb2 ⁺	tubulin alpha 2	HU, BLM, TBZ	W4C	[13, 36]
SPCC126.04c	sgf73 ⁺	histone acetyltransferase SAGA complex subunit	HU, MMS, TBZ	W4C	[12, 23]
SPCC126.15c	sec65 ⁺	signal recognition particle subunit	BLM, TBZ, UV	S4C	[12, 13]
SPCC162.12	tco89 ⁺	TORC1 subunit	HU, BLM, UV	NC	[37]
SPAC2F7.08c	snf5 ⁺	chromatin remodeling complex subunit	HU, MMS	1C	[38]
SPAC664.01c	swi6 ⁺	chromodomain protein	HU, TBZ	NC	[12, 13, 35]
SPAC19A8.11c		recombination protein	BLM	NC	[13]
SPAC3F10.02c	trk1 ⁺	potassium ion transporter	HU, BLM, CPT, TBZ, UV	2C	This study
SPAC1486.04c	alm1 ⁺	medial ring protein	HU, BLM, MMS, UV	NC	This study
SPAC17G6.06^d	rps2401 ⁺	40S ribosomal protein	HU, BLM, MMS, UV	2C	This study
SPBC2A9.02^e		NAD dependent epimerase/dehydratase family protein	HU, BLM, MMS, UV	1C	This study
SPCC63.02c	aah3 ⁺	alpha-amylase homolog	HU, BLM, TBZ, UV	W4C	This study
SPAC14C4.05c^d	mug61 ⁺	LEM domain protein, Sad1 interacting factor	HU, BLM, UV	2C	This study
SPAC1556.06^f	meu1 ⁺	meiotic expression up-regulated protein	HU, BLM, UV	NC	This study
SPAC22E12.11c	set3 ⁺	histone lysine methyltransferase	BLM, MMS, UV	W4C	This study
SPAC25H1.05^f	meu29 ⁺	meiotic expression up-regulated protein 29 precursor	HU, BLM, TBZ	W4C	This study
SPAC27D7.05c	apc14 ⁺	anaphase-promoting complex subunit	HU, TBZ, UV	NC	This study
SPAC3G6.01	hrp3 ⁺	ATP-dependent DNA helicase	BLM, TBZ, UV	2C	This study
SPBP8B7.13	vac7 ⁺	Vac7 ortholog	HU, MMS, TBZ	W4C	This study
SPCC830.06^d		calcineurin regulatory subunit	HU, BLM, TBZ	W4C	This study
SPAC27D7.08c^e		DUF890 family protein	HU, BLM	1C	This study
SPAC3F10.17^d		ribosome biogenesis protein	HU, BLM	2C	This study
SPBC29A10.02	mug12 ⁺	meiotic RNA-binding protein	HU, BLM	NC	This study
SPBC31E1.02c	pmr1 ⁺	P-type ATPase, calcium transporting	HU, UV	NC	This study
SPBC577.13^d	syj2 ⁺	inositol polyphosphate phosphatase	HU, TBZ	NC	This study
SPCC1494.03	arz1 ⁺	Zfs1 target number 1	MMS, UV	1C	This study
SPBC20F10.10^d	psl1 ⁺	cyclin pho85 family	MMS	NC	This study

^a See text for detailed descriptions of flow cytometry phenotypes.
^b Reference that reported involvement of gene in DDR.
^c NC, no change.
^d role inferred from homology.
^e conserved hypothetical.
^f sequence orphan.

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ISSN: 1471-2164

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