Figure 3

Characterization of ChIP-grade anti-PPARβ/δ antibody. (A) ChIP analysis for AcH4 or PPARβ/δ occupancy on chromatin from wild-type or Pparβ/δ-null keratinocytes treated with or without GW0742 (0.2 μM). qPCR was performed using chromatin immunoprecipitated with either an anti-AcH4 or the 8099 anti-PPARβ/δ antibody. (B) Integrated overview of microarray expression and ChIP-seq data for Angptl4. The region of the Angptl4 gene is shown in red on chromosome 17, and the corresponding genomic location of this gene is depicted below. Relative expression of Angptl4 is shown for the four treatment groups (wild-type and Pparβ/δ-null, with and without GW0742) with white representing basal expression, red representing greatly enhanced expression and pink representing enhanced expression. The exonic and intronic organization of the Angptl4 gene is depicted with boxes (exons) and lines (introns). Regions associated with increased PPARβ/δ occupancy based on ChiP-seq analysis (peaks) are shown in boxes corresponding to the genomic regions depicted above with the Z score listed below. Note the intronic region used for ChIP in (A), which is highlighted by arrows, is associated with a peak detected by ChIP-seq.