Figure 9

PPARβ/δ and ATF4 co-operatively modulate gene expression. (A) ChIP assays demonstrating promoter occupancy of both PPARβ/δ and ATF4 on representative type III target genes (Adrp, Prickle1 and Snai3) and one type V target gene (Angptl4). (B) Knockdown of ATF4 in SP1 cells. Upper panel shows mRNA analysis demonstrating effective knockdown of ATF4 by two of three shRNA vectors compared to control. Lower panel shows western blot analysis confirming ATF4 knockdown. (C) Effect of ATF4 knockdown on ligand activation of PPARβ/δ in SP1 cells. qPCR analysis of representative type III target genes (Adrp, Prickle1 and Snai3) and one type V target gene (Angptl4). Values represent the mean ± S.E.M. *Significantly lower ligand induction as compared to control as determined by ANOVA and post-hoc testing.