Production of BC
progeny for genetic linkage analysis. G. scabra breeding line SP6A1 is an unfixed breeding line, because most Japanese gentians exhibit inbreeding depression. Aki6PS is a double haploid line (DH) generated from G. triflora cv. ‘Ashiro-no-Aki’ using anther culture. Crossing the DH line Aki6PS back into one F1 individual yielded 93 BC1 progeny.