Table 2 Expression of protease genes in merozoites, gametocytes, unsporulated oocysts and sporulated oocysts of Eimeria tenella
From: Stage-specific expression of protease genes in the apicomplexan parasite, Eimeria tenella
Protease group | cDNA product | Stage specific expression | Predicted amplicon size | Actual amplicon size | Genbank accession number |
|---|---|---|---|---|---|
Aspartic Proteases | M G UO SO | Â | (bp) | (bp) | Â |
Eimepsin 1 |
| Oocyst Specific | 357 | 659 | AJ293829 |
Eimepsin 2 |
| Gametocyte Specific | 467 | 467 | JX503496 |
Eimepsin 3 |
| Merozoite Upregulated | 578 | 578 | JX503497 |
Cysteine proteases | Â | Â | Â | Â | Â |
Cathepsin B |
| Downregulated in Sporulated Oocyst | 656 | 656 | JN641867.1 |
Cathepsin L |
| Downregulated in Gametocytes | 330 | 300 | JX503498 |
Cathepsin C1 |
| Merozoite Upregulated | 547 | 547 | JX503499 |
Cathepsin C2 |
| Gametocyte Upregulated | 273 | 273 | JX503500 |
Cathepsin C3 |
| Gametocyte and Unsporulated Oocyst Upregulated | 436 | 436 | JX546580 |
Calpain |
| Merozoite Upregulated | 831 | 806 | JX503501 |
Ubiquitinyl hydrolase 1 |
| Merozoite Upregulated | 548 | 548 | JX503503 |
Ubiquitinyl hydrolase 2 |
| Gametocyte Upregulated | 395 | 395 | JX503504 |
Ubiquitinyl hydrolase 3 |
| Merozoite, possibly Gametocyte Upregulated | 698 | 698 | JX503505 |
Ubiquitinyl hydrolase 4 |
| Merozoite Upregulated | 499 | 499 | JX503506 |
Ubiquitinyl hydrolase 5 |
| Gametocyte Upregulated | 674 | 719 | JX503507 |
Ubiquitinyl hydrolase 6 |
| - | 546 | - | - |
Ubiquitinyl hydrolase 7 |
| - | 529 | - | - |
Ubiquitinyl hydrolase 8 |
| - | 303 | - | - |
OTU protease |
| - | 802 | - | - |
Pyroglutamyl peptidase |
| Gametocyte Upregulated | 735 | 771 | JX503502 |
Metalloproteases | Â | Â | Â | Â | Â |
Aminopeptidase N 1 |
| Downregulated in Merozoite | 602 | 602 | JX503508 |
Aminopeptidase N 2 |
| Gametocyte Upregulated | 636 | 636 | JX503509 |
ATP-dependant Zn protease 1 |
| Downregulated in Sporulated Oocyst | 604 | 604 | XM_001238661.1 |
ATP-dependant Zn protease 2 |
| Merozoite Upregulated | 389 | 389 | JX503516 |
ATP-dependant Zn protease 3 |
| Downregulated in Sporulated Oocyst | 567 | 1045 | JX503517 |
CAAX prenyl protease |
| Merozoite Upregulated | 368 | 368 | JX503518 |
Insulysin 1 |
| Merozoite Upregulated | 294 | 294 | JX503510 |
Insulysin 2 |
| Merozoite Upregulated | 416 | 416 | JX503511 |
Insulysin 3 |
| Oocyst Specific | 402 | 697 | JX503512 |
Insulysin 4 |
| Gametocyte Upregulated | 367 | 367 | JX503513 |
Insulysin 5 |
| Merozoite Upregulated | 202 | 202 | * |
Leucyl aminopeptidase |
| Merozoite, possibly Gametocyte Upregulated | 804 | 859 | JX503514 |
O-sialoglycoprotease |
| Merozoite Upregulated | 412 | 412 | JX503515 |
S2P-like protease |
| Gametocyte Specific | 616 | 1027 | JX503519 |
Serine proteases | Â | Â | Â | Â | Â |
Trypsin 1 |
| Gametocyte Specific | 613 | 613 | JX503520 |
Trypsin 2 |
| Gametocyte Upregulated | 721 | 721 | JX503521 |
Trypsin 3 |
| Merozoite Upregulated | 654 | 849 | JN987371.1 |
Subtilisin 1 |
| Gametocyte Specific | 260 | 260 | XM_001238722.1 |
Subtilisin 2 |
| Gametocyte Specific | 690 | 690 | XM_001238654.1 |
Subtilisin 3 |
| Merozoite Upregulated | 1171 | 1084 | JX503522 |
Subtilisin 4 |
| Gametocyte and Unsporulated Oocyst Upregulated | 566 | 566 | JX503523 |
Subtilisin 5 |
| Gametocyte Specific | 360 | 414 | JX503524 |
Subtilisin 6 |
| - | 365 | - | - |
Prolyl endopeptidase |
| Downregulated in Sporulated Oocyst | 534 | 534 | JX503526 |
Clp protease |
| Merozoite Upregulated | 413 | 241 | JX503525 |
Rhomboid protease 1 |
| Merozoite Upregulated | 554 | 554 | JN987619.1 |
