Table 3 Protease inhibitors used in the Eimeria tenella GAM56 processing assay
From: Stage-specific expression of protease genes in the apicomplexan parasite, Eimeria tenella
Inhibitor | Inhibitor target | Final concentration | Company |
|---|---|---|---|
Pepstatin A | Aspartyl | 1 μm | Sigma-Aldrich |
N-ethylmalemide | Cysteine | 1 mM | Sigma-Aldrich |
E-64 | Cysteine | 10 μM | MP Biomedicals |
Z-Phe-Ala-diazomethylketone | Cysteine | 2 μM | Bachem, UK |
Antipain | Ser + Cys | 100 μM | MP Biomedicals |
Chymostatin | Ser + Cys | 200 μg/ml | Sigma-Aldrich |
Leupeptin | Ser + Cys | 100 μM | Sigma-Aldrich |
AEBSF | Serine | 1 mM | Sigma-Aldrich |
Aprotinin | Serine | 0.002 TIU | MP Biomedicals |
Benzamidine HCl | Serine | 4 mM | Sigma-Aldrich |
Soybean Trypsin inhibitor | Serine | 100 μg/ml | Sigma-Aldrich |
Bestatin | Metallo (amino) | 40 μM | Sigma-Aldrich |
Phosphoramidon | Metallo (endo) | 10 μM | MP Biomedicals |
EDTA di-sodium salt | Metallo | 5 mM | Sigma-Aldrich |