High grade gliomas have varying levels of REST target gene expression. Grades III and IV gliomas from NCBI Dataset GSE4271 were broken into groups expressing high and low levels of REST signature genes (termed near-normal and REM tumors, respectively) using self-organizing maps and a euclidian distance metric. (A) Heatmap of REST signature gene expression for the 100 high-grade glioma tumors. (B) GSEA was used to determine if the differences in expression of REST target genes between REM and near-normal tumors are statistically significant. These results suggest that REM tumors indeed show significantly depleted expression of a wide range of REST target genes. GSEA parameters and genesets were utilized as in Figure 2.