Figure 4

Neuronal markers do not segregate with REM or near-normal tumors. (A) Box plot of neuronal markers derived from dataset GSE9566, which profiles genes expressed in pure populations of astrocytes, oligodendrocytes and neurons (Cahoy, Emery et al.[2008]). Genes that were selectively expressed in neurons (p<10^-15) were filtered out if they contained a consensus REST binding site within 30kb of the gene, or if REST had been published to bind the gene in ChIP-ChIP or ChIP-Seq experiments [3, 17]. Top, middle, and bottom lines of the box plot represent 75th, 50th and 25th percentile samples, respectively. The top and bottom whiskers represent 90th and 10th percentile samples, respectively. (B) Heatmap showing expression of neuronal markers in REM and near-normal tumors from gliomas in dataset GSE4271. Neither REM nor near-normal tumors show significant enrichment for non-REST associated neuronal markers. (C) Neither REM, Mid-, or Near Normal subtypes show a significant concerted increase in all non-REST target neuronal markers suggesting equal levels of neuronal involvement in the tumor.