Protein structure and positive selection in CSEP family 12. A: Amino acid alignment of the seven members obtained with CLC main workbench (see Methods). B: Evidence for selection on the paralog members of family 12 was estimated using the Selecton server ([49, 50];http://selecton.tau.ac.il/). Codon sites under positive diversifying (red) or purifying (purple and yellow) selection and conserved cysteines (yellow) are indicated by coloured circles. C: Cladogram with Ka/Ks-values indicated for the individual branches calculated using the on-line server athttp://services.cbu.uib.no/tools/kaks. Branches in red indicate a significant positive selection. D: 3D protein models of two family 12 members are shown and the amino acids under positive diversifying selection are highlighted in red.