Structure and evolution of barley powdery mildew effector candidates

BMC Genomics

Table 1 Summary of the 35 largest CSEP families

Family	Number of members	Motif¹⁾	C-term. cysteine²⁾	Conserved cysteines³⁾	Average peptide length⁴⁾	Haustoria/ Epiphytic exp-ratio⁵⁾	Preference for haustoria expression⁶⁾	Positive selection: Pairwise z-test⁷⁾	Positive selection: Average Ka/Ks-values⁸⁾
1	59		no	3	326	15	5	18 / 1711	0.68
2	32	FxC	no	4	396	6	0	3 / 496	0.73
3	20	F/YxC	no	10	384	6	0	0 / 190	0.70
4	19	FxC	yes (4–14)	2	153	58	16	8 / 171	1.20
5	15	F/Y/(H)xC	no	2	107	94	13	9 / 105	1.41
6	10	YxC	yes (1–9)	4	311	21	50	1 / 45	1.04
7	10		no	none	131	3	0	1 / 45	0.62
8	8	YxC	yes (4)	2	118	25	0	11 / 28	1.80
9	8		no	none	178	16	0	0 / 21	0.52
10	7	FxC	no	2	160	37	57	1 / 21	1.31
11	7		no	2	164	0	0	0 / 21	0.92
12	7	F/YxC	yes (10)	2	125	207	0	5 / 21	1.19
13	7	F/Y/(H)xC	yes (4)	2	123	30	14	9 / 21	1.69
14	7	YxC	no	2	127	0	0	0 / 21	0.50
15	7	YxC	no	4	310	3	0	1 / 21	0.82
16	6	FxC	no	2	144	55	0	2 / 15	1.59
17	6	F/YxC	no	5	395	11	0	0 / 15	0.56
18	6		no	2	153	74	50	0 / 15	0.97
19	5	F/YxC	yes (4)	2	115	87	0	1 / 10	0.93
20	5	FxC	yes (4)	2	128	168	0	0 / 10	0.92
21	5	WxC	yes (6)	2	118	156	20	9 / 10	2.76
22	5	YxC	yes (4)	2	105	31	20	2 / 10	2.00
23	5	YxC	yes (4)	2	107	197	20	8 / 10	2.61
24	4	F/YxC	yes (1–2)	2	125	403	50	1 / 6	0.80
25	4	YxC	yes (3–9)	2	132	168	25	3 / 6	1.56
26	4	YxC	yes (6)	8	392	6	0	0 / 6	0.68
27	4	F/YxC	no	3	162	10	0	0 / 6	0.90
28	4	FxC	no	5	373	5	0	0 / 6	0.83
29	4	FxC	yes (5–7)	2	115	67	0	3 / 6	2.09
30	4	YxC	yes (4)	3	121	101	75	4 / 6	2.45
31	4	YxC	yes (4–7)	2	119	141	25	0 / 6	1.37
32	4	F/YxC	yes (4)	2	112	168	0	2 / 6	1.35
33	4	YxC	no	2	150	0	0	0 / 6	1.03
34	4		no	none	122	369	25	1 / 6	1.68
35	4		no	4	126	not calculated	25	0 / 6	1.07

The 35 CSEP families with four or more members. The table summarizes data from gene- and protein expression as well as protein length, conserved cysteines, YxC-motif and detection of positive selection within each family. Data of families showing positive selection in the overall z-test are indicated in bold fonts. A table with more extensive details of the CSEP family analysis is provided in the Additional Files (Additional file1). 1) Indicates the type motif in the N-terminus of the mature protein 2) The presence of a cysteine close to the C-terminus and the distance to the C-terminus is indicated in brackets 3) Number of conserved cysteines in the mature protein. 4) Length of proteins (number of amino acid residues): The average lengths of the proteins were calculated for each family. 5) Gene expression ratio in haustorial samples versus epiphytic samples, calculated as averages for each family. 6) Percentages of CSEPs in each family found only in haustoria-samples by proteome analysis. 7) Numbers of pairs with significant positive selection (z-tests at 5% level) compared to the total number of pairs within each family. 8) Family averages of mean Ka/Ks-values calculated on the mature proteins.

ISSN: 1471-2164