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Table 1 Summary of the 35 largest CSEP families

From: Structure and evolution of barley powdery mildew effector candidates

Family Number of members Motif1) C-term. cysteine2) Conserved cysteines3) Average peptide length4) Haustoria/ Epiphytic exp-ratio5) Preference for haustoria expression6) Positive selection: Pairwise z-test7) Positive selection: Average Ka/Ks-values8)
1 59   no 3 326 15 5 18 / 1711 0.68
2 32 FxC no 4 396 6 0 3 / 496 0.73
3 20 F/YxC no 10 384 6 0 0 / 190 0.70
4 19 FxC yes (4–14) 2 153 58 16 8 / 171 1.20
5 15 F/Y/(H)xC no 2 107 94 13 9 / 105 1.41
6 10 YxC yes (1–9) 4 311 21 50 1 / 45 1.04
7 10   no none 131 3 0 1 / 45 0.62
8 8 YxC yes (4) 2 118 25 0 11 / 28 1.80
9 8   no none 178 16 0 0 / 21 0.52
10 7 FxC no 2 160 37 57 1 / 21 1.31
11 7   no 2 164 0 0 0 / 21 0.92
12 7 F/YxC yes (10) 2 125 207 0 5 / 21 1.19
13 7 F/Y/(H)xC yes (4) 2 123 30 14 9 / 21 1.69
14 7 YxC no 2 127 0 0 0 / 21 0.50
15 7 YxC no 4 310 3 0 1 / 21 0.82
16 6 FxC no 2 144 55 0 2 / 15 1.59
17 6 F/YxC no 5 395 11 0 0 / 15 0.56
18 6   no 2 153 74 50 0 / 15 0.97
19 5 F/YxC yes (4) 2 115 87 0 1 / 10 0.93
20 5 FxC yes (4) 2 128 168 0 0 / 10 0.92
21 5 WxC yes (6) 2 118 156 20 9 / 10 2.76
22 5 YxC yes (4) 2 105 31 20 2 / 10 2.00
23 5 YxC yes (4) 2 107 197 20 8 / 10 2.61
24 4 F/YxC yes (1–2) 2 125 403 50 1 / 6 0.80
25 4 YxC yes (3–9) 2 132 168 25 3 / 6 1.56
26 4 YxC yes (6) 8 392 6 0 0 / 6 0.68
27 4 F/YxC no 3 162 10 0 0 / 6 0.90
28 4 FxC no 5 373 5 0 0 / 6 0.83
29 4 FxC yes (5–7) 2 115 67 0 3 / 6 2.09
30 4 YxC yes (4) 3 121 101 75 4 / 6 2.45
31 4 YxC yes (4–7) 2 119 141 25 0 / 6 1.37
32 4 F/YxC yes (4) 2 112 168 0 2 / 6 1.35
33 4 YxC no 2 150 0 0 0 / 6 1.03
34 4   no none 122 369 25 1 / 6 1.68
35 4   no 4 126 not calculated 25 0 / 6 1.07
  1. The 35 CSEP families with four or more members. The table summarizes data from gene- and protein expression as well as protein length, conserved cysteines, YxC-motif and detection of positive selection within each family. Data of families showing positive selection in the overall z-test are indicated in bold fonts. A table with more extensive details of the CSEP family analysis is provided in the Additional Files (Additional file1). 1) Indicates the type motif in the N-terminus of the mature protein 2) The presence of a cysteine close to the C-terminus and the distance to the C-terminus is indicated in brackets 3) Number of conserved cysteines in the mature protein. 4) Length of proteins (number of amino acid residues): The average lengths of the proteins were calculated for each family. 5) Gene expression ratio in haustorial samples versus epiphytic samples, calculated as averages for each family. 6) Percentages of CSEPs in each family found only in haustoria-samples by proteome analysis. 7) Numbers of pairs with significant positive selection (z-tests at 5% level) compared to the total number of pairs within each family. 8) Family averages of mean Ka/Ks-values calculated on the mature proteins.