Table 3 GenBank accession numbers, PCR primers, amplicon sizes and PCR efficiencies of the RT-qPCR assays used in the current experiment
Gene | Accession no. | Forward primer | Reverse primer | Amplicon size(bp) | PCR efficiency |
|---|---|---|---|---|---|
CYP1A | EX725014 | CCTTGACCTCTCGGAGAAAGAC | CGCCCCGCTAGCTATAGACA | 146 | 1.85 |
CYP1B1 | GmE090818c16636 | CAGATGCCCCACATCACCTT | GATCGCCTTGTCTCCGTTCA | 108 | 2.10 |
CYP1C1 | GmE100215i31189 | GTCAACCAGTCGTCCGTGAA | CGTTGTTCGTGAGGTCCTTGT | 115 | 2.02 |
AhR2 | EX728781 | CAACCGGCGGTCCACAT | GCACCATGCAGTTGCCAGTA | 132 | 2.08 |
AHRR | F2Z8C0H01CDEIU | GAGGTGTTTGTCTCCCGTCTCT | TTCACCAGGTCCACGCAGTA | 77 | 2.06 |
GST pi | EX730032 | GTCCCCCTGCTGCCATTC | CCTCCATACACCGCCACCTA | 126 | 1.85 |
p53 | EX723548 | CGCTGCTGCTGAACTTCATG | GGATGGCTCTCCGGTTCAT | 63 | 1.86 |
RAD51 | AACCTAGCATCCCGCTCAAA | TGGTCAGGACAACTGGGATGT | 91 | 1.89 | |
IGF1 | CTCTCCAAATCCGTCTCCTGTT | AGATGCGGGCAATGTCACAT | 101 | 1.99 | |
IGFBP1 | GTGAGTCCTCCCTGGATGGA | CTCGACCCCAGGATCTTCTTC | 79 | 2.02 | |
Ferritin | EX726260 | GCGTCTAGCTGGACCCCTTA | GGGCGACTACATCAGCAACC | 101 | 2.08 |
Transferrin | EX722617 | ACGACGGGTCAACTCGTTTG | AGTCACTCGGGCACAATGTG | 139 | 1.83 |
HSP70 | EX741726 | CATGACATCGTCCTGGTTGGT | CGTAGGCCACAGCTTCATCA | 121 | 1.84 |
HPX (WAP65-2) | GmE100215i27905 | CGCCTGCTCTACATCGTCAA | CGTAGCGTCCAGTGGTCCTT | 129 | 1.89 |
MCM2 | GmE100215i14387 | TGGAGGACGACGTGAACATG | TCGTTGTTGTCGCGTTTGAA | 130 | 1.77 |
CDCA7 | GmE100215i27789 | GCATCTGCACCAACGTGAAG | CCCCCACACACTCTGGGTTA | 120 | 2.02 |
EF1A | EX722124 | CGGTATCCTCAAGCCCAACA | GTCAGAGACTCGTGGTGCATCT | 93 | 1.97 |
B-actin | EX739174 | CACAGCCGAGCGTGAGATT | ACGAGCTAGAAGCGGTTTGC | 95 | 1.93 |
Ubiquitin | EX735613 | GGCCGCAAAGATGCAGAT | CTGGGCTCGACCTCAAGAGT | 69 | 1.93 |
RPL4 | EX725958 | GGTGCCATACAGCTGATCCA | CCAGGCATCACACTGCAGAA | 123 | 1.83 |