Proposed strategy to optimize bait usage for maximal coverage of target regions while minimizing the size of the bait pool. Solid lines indicate bait placement, and dotted lines indicate regions for which acceptable depth of coverage is expected in the absence of bait coverage. For target regions less than 300bp, a single bait is placed centered on the region. For targets greater than 300bp, baits are tiled with ~150bp gaps between adjacent baits. When two target regions are separated by less than 150bp, a contiguous sequence is expected to be recovered in the absence of bait coverage between the two regions.