Confirmation of FOXP3 expression in transfected Jurkat T cells. (A) Quantitative real-time RT-PCR for FOXP3 expression. Bars represent mean ± SD of duplicate measurements. (B) FACS analysis of intracellular FOXP3 staining. (C) Analysis of cell lysates from equal numbers of Jurkat cells followed by FOXP3-specific Western blot analysis. All data are representative of at least two independent experiments.