Most HDACs and sirtuins are constitutively expressed in H9c2 rat cardiac myocytes and TSA treatment does not affect their expression. Total cell extracts from control (C) and trichostatin treated cells (TSA) were probed with monospecific antibodies to the various enzymes as noted. Nuclear (Nu) and cytoplasmic (Cy) distribution of HDACs and sirtuins were similarly probed. The data are shown for untreated cells only. TSA-treatment did not change the subcellular distributions (Data not shown).