HDAC inhibitors affect phosphorylation of ERK and p38 in H9c2 cells. Cells were incubated with or without TSA or CBHA at different time intervals as shown. Ten microgram aliquots of protein extracts were separated by 10% SDS-PAGE. Western blots were probed with mono-specific antibodies against phospho-ERK or p38 MAPK. Blots were stripped, washed and re-probed with anti-ERK and p38 antibodies. Representative western blots of ERK and p38 MAPK in response to TSA and CBHA are shown.