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Table 1 Quantitative PCR measurements of the steady state levels of various HDACs and sirtuins in H9c2 cells *

From: Pan-histone deacetylase inhibitors regulate signaling pathways involved in proliferative and pro-inflammatory mechanisms in H9c2 cells

Enzyme δCp ( Mean ±SD ) Relative expression
HDAC 1 1.36 ± 0.06 3.9
HDAC 2 0.2 ± 0.004 26.4
HDAC 3 2.26 ± 0.25 2.3
HDAC 4 5.28 ± 0.24 1
HDAC 5 4.92 ± 0.95 1.1
HDAC 6 3.43 ± 0.13 1.5
HDAC 7 2.81 ± 0.28 1.9
Sirtuin 1 6.06 ± 0.38 1.4
Sirtuin 2 2.17 ± 0.38 3.9
Sirtuin 3 6 .00 ± 0.38 1.4
Sirtuin 4 8.43 ± 0.39 1
Sirtuin 5 3.35 ± 0.29 2.5
Sirtuin 6 5.07 ± 0.38 1.7
Sirtuin 7 5.75 ± 0.59 1.5
  1. * qPCR was carried out on total RNA extracted from H9c2 cells grown in serum free or serum supplemented media. Expression levels were calculated from δCp values from 3 replicate determinations. Expression levels of various HDACs were compared to HDAC 4 fixed at 1: similarly fold-changes in the expression of sirtuins were established against sirtuin 4 whose expression was fixed at 1.