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Table 1 Quantitative PCR measurements of the steady state levels of various HDACs and sirtuins in H9c2 cells *

From: Pan-histone deacetylase inhibitors regulate signaling pathways involved in proliferative and pro-inflammatory mechanisms in H9c2 cells

Enzyme

δCp ( Mean ±SD )

Relative expression

HDAC 1

1.36 ± 0.06

3.9

HDAC 2

0.2 ± 0.004

26.4

HDAC 3

2.26 ± 0.25

2.3

HDAC 4

5.28 ± 0.24

1

HDAC 5

4.92 ± 0.95

1.1

HDAC 6

3.43 ± 0.13

1.5

HDAC 7

2.81 ± 0.28

1.9

Sirtuin 1

6.06 ± 0.38

1.4

Sirtuin 2

2.17 ± 0.38

3.9

Sirtuin 3

6 .00 ± 0.38

1.4

Sirtuin 4

8.43 ± 0.39

1

Sirtuin 5

3.35 ± 0.29

2.5

Sirtuin 6

5.07 ± 0.38

1.7

Sirtuin 7

5.75 ± 0.59

1.5

  1. * qPCR was carried out on total RNA extracted from H9c2 cells grown in serum free or serum supplemented media. Expression levels were calculated from δCp values from 3 replicate determinations. Expression levels of various HDACs were compared to HDAC 4 fixed at 1: similarly fold-changes in the expression of sirtuins were established against sirtuin 4 whose expression was fixed at 1.