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Table 1 Semi-quantitative analysis of SCGB1A1 RT-PCR amplicons

From: Multiple secretoglobin 1A1 genes are differentially expressed in horses

   Relative amplicon density
Expression category Tissue GAPDH SCGB 1A1 SCGB 1A1A n
High Lung 1.00 3.50 ± 0.94 3.65 ± 0.72 5
  Uterus 1.00 2.25 ± 0.59 2.02 ± 0.61 4
  Fallopian tube 1.00 2.67 ± 1.60 1.49 ± 1.59 3
  Mammary gland 1.00 0.67 ± 0.67 nd - 0.33 3
  Ovary 1.00 nd - 0.65 nd - 0.07 3
  Prostate 1.00 nd - 1.67 nd 2
Low Aorta 1.00 0.42 ± 0.55 nd - 0.32 4
  Urethra 1.00 0.25 ± 0.38 0.01 ± 0.01 4
  Epididymis 1.00 nd - 0.07 0.16 ± 0.20 2
  Skin 1.00 nd - 0.49 0.04 ± 0.01 4
  Trachea 1.00 nd - 0.19 0.81 ± 0.73 4
  Eye 1.00 nd - 0.26 nd - 0.43 4
  Brain 1.00 0.09 ± 0.08 0.08 ± 0.02 4
  Nose epithelium 1.00 nd - 0.17 0.04 ± 0.03 4
  Bladder 1.00 0.06 ± 0.04 nd - 0.03 4
  Spleen 1.00 nd - 0.07 nd - 0.04 4
  Kidney 1.00 nd - 0.02 nd - 0.01 4
  Liver 1.00 nd - 0.02 nd - 0.01 4
  Tongue 1.00 nd - 0.05 nd 3
  Small intestine 1.00 0.02 ± 0.01 nd 4
  Large intestine 1.00 0.03 ± 0.02 nd 4
  Pituitary 1.00 nd - 0.01 nd - 0.02 4
  Salivary gland 1.00 nd - 0.01 nd 4
  Adrenal gland 1.00 nd - 0.01 nd 4
  Seminal vesicle 1.00 nd - 0.42 nd 2
  Testis 1.00 nd 0.02 ± 0.01 2
Absent Eyelid gland 1.00 nd nd 4
  Cardiac muscle 1.00 nd nd 4
  Thyroid 1.00 nd nd 4
  Stomach 1.00 nd nd 4
  Pancreas 1.00 nd nd 4
  Lymph node 1.00 nd nd 4
  Bone marrow 1.00 nd nd 3
  1. Quantification of gene-specific SCGB1A1 cDNA amplicon intensity relative to GAPDH using Image Lab™ software 2.0.1; nd, non-detected.