Figure 2

Typical domain architectures of Ribonuclease III and Pasha proteins. Members of the Ribonuclease III (RNase III) protein family all contain RNase III protein domains responsible for binding Mg²⁺ ions that cleave individual strands of dsRNA. The dsRNA binding domain (dsRBD) is common to most RNase III proteins and Pasha. Other common domains found in RNase III class 3 (Dicer) proteins include PAZ, a domain of unknown function (DUF), and a helicase. Pasha contains only tandem dsRBD domains, a domain architecture relatively common in other dsRNA binding proteins within metazoan proteomes.