Chromosome rearrangements observed in the genome of breast cancer cell line ZR-75-30. A. Genome-wide Circos plot of structural variation in the ZR-75-30 genome. An ideogram of a normal karyotype is shown around the outside. Copy number variation is represented by the blue line, shown inside the ideogram. Chromosome rearrangements are depicted with green (interchromosomal) and purple (intrachromosomal) lines. All 125 structural variants shown have been independently validated by PCR (red lines) or by matching to a copy number step on a SNP6 array. B. Structural variation in the complex 8;17 amplicon. Colours as in A. C. Copy number variation in the 8;17 amplicon of ZR-75-30. Ideograms of chromosomes 8 and 17 are shown, with regions containing amplified segments highlighted with a red box and expanded below. Copy number changes, as measured by paired-end sequencing, illustrate the complexity of the amplification. An example set of ten confirmed rearrangement junctions are shown with green (interchromosomal) and purple (intrachromosomal) lines. Genome positions are based on Hg19.