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Table 3 Sequencing coverage

From: Reference genome-independent assessment of mutation density using restriction enzyme-phased sequencing

Reference Read Alignment Control T1 T2 T3
None Total number of reads (million) 15.5 13.7 28.5 29.9
O.s. 6.11 Number of mapped reads (million) 13.4 11.8 24.6 26.4
O.s. 6.1 Complexity (Mb) 131.8 131.5 142.8 142.4
O.s. 6.1 Complexity, homozygous changes2 (Mb) 78.2 77.7 82.7 82.6
De novo3 Number of mapped reads (million) 13.3 11.7 24.5 26.3
De novo Complexity (Mb) 102.4 102.1 106.9 106.7
De novo Complexity, homozygous changes2 (Mb) 82.6 82.1 87.4 87.3
  1. 1Reference complexity: 380 Mb. 2Complexity for homozygous scoring is calculated according to the following requirements: i) covered at least once in each of the four libraries, ii) covered at least twice in the putative mutant, iii) covered less than 200 times cumulatively in all libraries. 3Reference complexity: 117 Mb.