Reference genome-independent assessment of mutation density using restriction enzyme-phased sequencing

BMC Genomics

Table 3 Sequencing coverage

Reference	Read Alignment	Control	T1	T2	T3
None	Total number of reads (million)	15.5	13.7	28.5	29.9
O.s. 6.1¹	Number of mapped reads (million)	13.4	11.8	24.6	26.4
O.s. 6.1	Complexity (Mb)	131.8	131.5	142.8	142.4
O.s. 6.1	Complexity, homozygous changes² (Mb)	78.2	77.7	82.7	82.6
De novo³	Number of mapped reads (million)	13.3	11.7	24.5	26.3
De novo	Complexity (Mb)	102.4	102.1	106.9	106.7
De novo	Complexity, homozygous changes² (Mb)	82.6	82.1	87.4	87.3

¹Reference complexity: 380 Mb. ²Complexity for homozygous scoring is calculated according to the following requirements: i) covered at least once in each of the four libraries, ii) covered at least twice in the putative mutant, iii) covered less than 200 times cumulatively in all libraries. ³Reference complexity: 117 Mb.

ISSN: 1471-2164