Genome-guided analysis of physiological and morphological traits of the fermentative acetate oxidizer Thermacetogenium phaeum

Table 1 Growth of Thermacetogenium phaeum with different substrates in pure and syntrophic co-culture

Pure culture	ΔOD₆₀₀	Cell density [mg · l^-1]	Substrate utilized [mM]	Acetate formed [mM]	electron balance [%]	Molar growth yield Y_E [g · mol^-1]
Acetoin	0.05	12.5	7.3	10.5	97.5	1.71
2,3 Butanediol	0.046	11.5	4.5	8.3	151.3	2.56
Methanol	0.583	145.8	12.2	7	86.5	11.95
Ethanol	0.047	11.75	15.3	17.2	75.5	0.77
Syntrophic culture
Acetoin	0.377	94.25	7.4	12.5	128.5	12.74
2,3 Butanediol	0.045	11.25	7.7	11.4	120.1	1.46
Methanol	0.02	5	0.2	0.2	-	-
Ethanol	0.07	17.5	20.2	15.7	52.3	0.87

Molar growth yields (Y) and electron balance were calculated using the formula <C₄H₇O₃> for cell material and the experimentally determined conversion factor OD600 = 1 = 0.250 g cell mass· L^-1.
Data show growth after 4 weeks. In most cases no further growth could be observed.

ISSN: 1471-2164