HK2-targeting miRNAs regulate the expression of HK2 protein. HK2 and β-actin protein expression was determined by western blotting. Mac-T cells were transfected with HK2-targeting miRNAs and a negative control (NC). Cell lysates were collected after 24 hours and analyzed by western blotting. Beta-actin served as the internal control. The protein fragment intensity was quantified using Imagpro-Plus software, and IOD was used to represent the protein level. Error bars represent SD. n=3. a-c means different superscripts differ (P<0.05).