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Table 2 Validation of the SNP scoring scheme by PCR-based re-sequencing

From: A genomic scale map of genetic diversity in Trypanosoma cruzi

 

SNP score range

Total

 

0–0.4

0.4–0.7

0.7–1.0

(0–1)

 

V

NV

V

NV

V

NV

V

NV

No. of SNPs

9

63

25

8

996

35

1030

106

Percent validation

12.5

75.76

96.61

90.67

  1. Fragments from selected loci were amplified by PCR and directly sequenced, and SNPs were identified using PolyPhred after base-calling. The table shows the number of SNPs identified in silico that have been validated (V) or not validated (NV), grouped by their score.