Figure 3

Density distribution of FNR loci along the reads. The density distribution of false negative SNPs was calculated and plotted. A large proportion of false negative SNPs located in the first five bases of each read, indicating great influence of disruption of recognition site. The inset shows the magnified distribution from position 6 to 90 along read and the dashed vertical line represents the position 55 after which the number of false negative loci increased sharply.