Figure 5

Phylogenetic analysis of Tasmanian devil MHC Class I sequences variants. The phylogenetic relationship was inferred using the Neighbour-Joining method [46]. The percentage of replicate trees in which the associated sequences clustered together in the bootstrap test (1,000 replicates) are displayed next to the branches, indicating the level of reliability of the phylogeny [47]. Bootstrap frequencies lower than 50% are not shown. Phylogenetic analysis was conducted in MEGA5 [45]. Modo-UK [GenBank:EU886686] and Maeu-UK [GenBank:CU463018] are orthologous UK genes in the grey short-tailed opossum and tammar wallaby. Modo-UA [GenBank:DQ067089], UB [GenBank:NM_001079820] and UC [GenBank:NM_001079819] are Class I genes in the opossum.