Figure 3

Amount of overlap between complete transcripts identified in different assemblies of P . fastigiatum reads. 380 different assemblies were conducted using ABySS [25, 26] and a combination of (i) coverage cutoffs between 2 and 20 and (ii) k-mer sizes between 25 and 63. Transcripts covering a complete coding sequence of the homologue from A. lyrata or A. thaliana were identified and counted. These were searched against each other and the number of sequences present in each of two assemblies was determined. The number of overlapping sequences was then divided by the sum of the number of sequences identified in both assemblies and again divided by 0.5 to obtain values between 0 and 1. These percentages were colour coded with yellow representing high values and darker colours low values. A black box means no overlap between the sets of complete transcripts.