A low protein diet during pregnancy provokes a lasting shift of hepatic expression of genes related to cell cycle throughout ontogenesis in a porcine model

Table 4 Comparison of microarray data and qRT-PCR of selected transcripts

Gene name	Microarray			qRT-PCR #			Correlation ##
	p-value	FC	Regulation	p-value	FC	Regulation	Expr. values
94 dpc
CCND2	0.022	-1.25	down	0.0001	-1.83	down	0.47 *
NCAPG	0.019	-1.73	down	0.003	-1.58	down	0.47 *
MGMT	0.031	+1.12	up	0.011	+1.32	up	0.53 **
GADD45B	0.669	-1.08	n.r.	0.522	+1.17	n.r	0.68 ***
1 dpn
SDHB	0.0006	-1.19	down	0.028	-1.30	down	0.71 ***
CCND2	0.711	-1.06	n.r.	0.651	-1.09	n.r.	0.46 *
PPARGC1A	0.745	-1.03	n.r.	0.761	-1.04	n.r.	0.67 ***
188 dpn
PRKAA1	0.0004	-1.68	down	0.027	-1.40	down	0.77 ***
PRKAA2	0.009	-1.36	down	0.095	-1.25	down	0.10
PPARGC1A	0.126	-1.35	n.r.	0.105	-1.36	n.r.	0.86 ***

CCND2 - cyclin D2; NCAPG - non-SMC condensin I complex, subunit G; MGMT - O-6-methylguanine-DNA methyltransferase; GADD45B - growth arrest and DNA-damage-inducible, β ; SDHB - succinate dehydrogenase complex, subunit B, iron sulfur (Ip); PPARGC1A - Peroxisome proliferator activated receptor γ coactivator-1α; PRKAA1 - 5'-AMP-activated protein kinase, catalytic α 1 chain; PRKAA2 - 5'-AMP-activated protein kinase, catalytic α 2 chain *calculated by factorial normalisation on RPL10 expression values; **p-value of pamn' rho; n.r. - not regulated

ISSN: 1471-2164