A low protein diet during pregnancy provokes a lasting shift of hepatic expression of genes related to cell cycle throughout ontogenesis in a porcine model

Table 5 Primer used to verify microarray experiments in liver tissue by qRT-PCR

Gene name	Probe-set ID	Sequence 5' - 3'	T(°C)	Size (bp)
CCND2	Ssc.15749.1.S1_at	For AGGAGCAGATTGAGGTCGTG	86	185
		Rev CAACCAGAGAGAAGGAAGGAGA
NCAPG	Ssc.28512.1.S1_at	For CTTGTAGATTTGACGAGACCA	60	156
		Rev GGCTTTAGTATAGACCCGAAC
MGMT	Ssc.19639.1.A1_at	For GCAACTACTCGGGAGGAATG	88	171
		Rev CTGCGAACGCTCAGTCTTG
GADD45B	Ssc.14764.1.A1_at	For GGACTTAGACTTTGGGACTTG	60	140
		Rev GTAAGCCTCCCATCTCTCTT
SDHB	Ssc.8939.1.S1_at	For GCAGGACCCGTTCTCTCTGT	60	170
		Rev GGTTACAGTCACGTTAGGTTGG
PPARGC1A	Ssc.16864.1.S1_at	For GTAAATCTGCGGGATGATGG	60	208
		Rev TGGTGGAAGCAGGATCAAAG
PRKAA1	Ssc.8107.1.A1_at	For TTGTTAATTTCATAAACTTTGCTTC	60	193
		Rev GTGCAGCCTTGACATACTC
PRKAA2	Ssc.16257.1.S1_at	For TCTGTAATTCTGTTTTGCCTACGA	60	168
		Rev AGCAAGAAGGTGATGCCAAG
RPL10*	Ssc.9130.1.A1_at	For CTGTGTTCGTCTTTTCTTCC	60	199
		Rev TCATCCACTTTTGCCTTCT

CCND2 - cyclin D2; NCAPG - non-SMC condensin I complex, subunit G; MGMT - O-6-methylguanine-DNA methyltransferase; GADD45B - growth arrest and DNA-damage-inducible, β ; SDHB - succinate dehydrogenase complex, subunit B, iron sulfur (Ip); PPARGC1A - Peroxisome proliferator activated receptor γ coactivator-1α; PRKAA1 - 5'-AMP-activated protein kinase, catalytic α 1 chain; PRKAA2 - 5'-AMP-activated protein kinase, catalytic α 2 chain; RPL10 - Ribosomal protein 10; * house keeping gene.

ISSN: 1471-2164