miRNA arm selection and isomiR distribution in gastric cancer

BMC Genomics

Table 1 Summary of analysis on miRNA reads

	G1245N	G1245T
# Reads	17,492,248	23,974,839
# detected pre-miRNAs ^a	542	556
# detected miRNAs ^a	688	705
# miRNAs at opposite arm ^b	87	96
# isomiRs	5,132	5,305
# isomiRs with length equal to miRNA	542	569

We mapped the sequence reads back to human pre-miRNA, quantifying the expression levels of pre-miRNAs, mature miRNA and isomiRs.
^a: The human pre-miRNA and mature miRNA are based on the definition of miRBase 16. Totally, there are 1,048 pre-miRNAs and 1,223 mature miRNAs, resulting in 1,366 miRNA/pre-miRNA pairs.
^b: According to miRBase 16, 730 out of 1,048 human pre-miRNAs encode only one mature miRNA at one arm, either 5p or 3p arm. With the improvement on NGS's sequencing intensity, we detected additional opposite-arm mature miRNAs at the pre-miRNAs originally encoding only one miRNA.

ISSN: 1471-2164