Figure 2

Time course response to lapatinib by HCT116 with reporter for MKI 67: Left panels show 2 typical fluorescent images (nuclei: blue, GFP: green) sampled for the same site in a 48-hour lapatinib treatment. a) The upper panels show the case before any drug is applied. b) The lower panels show the case 48 hours after lapatinib was added. The right panels show the log2(GFP) intensity histogram for each time point. The fraction of the total population having a particular intensity is shown on the y-axis and the log2 intensity of the eGFP fluorescence measured for the cell is shown on the x-axis. The distribution before drug is shown as a thick yellow line at the upper right panel. The lower right panel shows the profile that is color-coded with time, starting with red, changing to yellow and then green, and ending with blue. The profile at the ending time points is shown with bold yellow line.