Computational discovery and RT-PCR validation of novel Burkholderia conserved and Burkholderia pseudomallei unique sRNAs

Table 1 Discovery and verification of bacterial sRNAs in previous studies.

Bacteria	Computational discovery method	Verification method	Number of sRNAs			Reference
			Predicted	Tested	Verified
Escherichia coli	QRNA	Northern blot	275	49	11	[64]
Escherichia coli	Pftools2.2 & RNAMotif	Northern blot	227	8	7	[65]
Burkholderia cenocepacia	QRNA	Microarray	3,441	213	4	[39]
Streptomyces coelicolor	BLAST & TransTermHP	RT-PCR & microarray	37	32	20	[66]
Synechocystis PCC6803	RNAz	Northern blot	383	2	2	[67]
Staphylococcus aureus	RNAsim	Northern blot	774	36	11	[68]
Escherichia coli	Anonymous program	Northern blot	601	6	3	[69]
Salmonella enterica Typhimurium	sRNAscanner	Northern blot	156	16	6	[46]
Streptomyces coelicolor	RNAz & nocoRNAc	Microarray	843	403	317	[47]
Burkholderia pseudomallei	SIPHT, sRNAscanner & Rfam_scan	RT-PCR	1306	15	8	This study.

ISSN: 1471-2164