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Table 3 Validation of differentially expressed miRNAs using PCR.

From: Comprehensive analysis of microRNAs in breast cancer

miRNA NGS normal NGS tumor NGS fc PCR fc Consistent?
has-let-7b 32 594.1 16 107.1 0.49 0.46 Y
hsa-let-7c 7 710.1 2 439.1 0.32 0.44 Y
hsa-miR-22 69 532.1 24 576.1 0.35 0.24 Y
hsa-miR-125b 44 624.1 6 613.1 0.15 0.37 Y
hsa-miR-143 38 419.1 17 359.1 0.45 0.14 Y
hsa-miR-144 3 548.1 351.1 0.10 0.30 Y
hsa-miR-145 28 682.1 6 435.1 0.22 0.26 Y
hsa-miR-193a-5p 1 836.1 298.1 0.16 0.27 Y
hsa-miR-193b 2 469.1 1 058.1 0.43 0.30 Y
hsa-miR-199b-5p 1 608.1 1 055.1 0.66 0.83 Y
hsa-miR-320a 16 734.1 3 060.1 0.18 0.75 Y
hsa-miR-378 12 610.1 1 542.1 0.12 0.24 Y
hsa-miR-497 8 005.1 2 010.1 0.25 0.29 Y
hsa-miR-99a 31 360.1 5 548.1 0.18 0.16 Y
hsa-miR-141 5007.1 14 541.1 2.90 5.88 Y
hsa-miR-375 240.1 2 372.1 9.88 37.95 Y
hsa-miR-425 592.1 2 529.1 4.27 1.93 Y
hsa-miR-203 197.1 1 045.1 5.30 9.80 Y
hsa-miR-342-3p 350.1 1 331.1 3.80 3.43 Y
hsa-miR-182 316.1 1 653.1 5.23 11.86 Y
hsa-miR-200b 1 362.1 4 162.1 3.06 12.61 Y
hsa-miR-183 118.1 1 067.1 9.04 6.12 Y
hsa-miR-423-3p 2 446.1 1 993.1 0.81 5.96 N
  1. NGS normal and NGS tumor denote miRNA relative read counts (TPM) in normal and tumor tissues. The NGS fold change (NGS fc) values are the quotients of miRNA read counts in normal tissue divided by those in tumor tissue. The PCR fold change (PCR fc) values are the quotients of miRNA expression levels, relative to U6 in normal tissue, divided by those in tumor tissue, averaged from 4 independent experiments