Table 3 Validation of differentially expressed miRNAs using PCR.
miRNA | NGS normal | NGS tumor | NGS fc | PCR fc | Consistent? |
|---|---|---|---|---|---|
has-let-7b | 32 594.1 | 16 107.1 | 0.49 | 0.46 | Y |
hsa-let-7c | 7 710.1 | 2 439.1 | 0.32 | 0.44 | Y |
hsa-miR-22 | 69 532.1 | 24 576.1 | 0.35 | 0.24 | Y |
hsa-miR-125b | 44 624.1 | 6 613.1 | 0.15 | 0.37 | Y |
hsa-miR-143 | 38 419.1 | 17 359.1 | 0.45 | 0.14 | Y |
hsa-miR-144 | 3 548.1 | 351.1 | 0.10 | 0.30 | Y |
hsa-miR-145 | 28 682.1 | 6 435.1 | 0.22 | 0.26 | Y |
hsa-miR-193a-5p | 1 836.1 | 298.1 | 0.16 | 0.27 | Y |
hsa-miR-193b | 2 469.1 | 1 058.1 | 0.43 | 0.30 | Y |
hsa-miR-199b-5p | 1 608.1 | 1 055.1 | 0.66 | 0.83 | Y |
hsa-miR-320a | 16 734.1 | 3 060.1 | 0.18 | 0.75 | Y |
hsa-miR-378 | 12 610.1 | 1 542.1 | 0.12 | 0.24 | Y |
hsa-miR-497 | 8 005.1 | 2 010.1 | 0.25 | 0.29 | Y |
hsa-miR-99a | 31 360.1 | 5 548.1 | 0.18 | 0.16 | Y |
hsa-miR-141 | 5007.1 | 14 541.1 | 2.90 | 5.88 | Y |
hsa-miR-375 | 240.1 | 2 372.1 | 9.88 | 37.95 | Y |
hsa-miR-425 | 592.1 | 2 529.1 | 4.27 | 1.93 | Y |
hsa-miR-203 | 197.1 | 1 045.1 | 5.30 | 9.80 | Y |
hsa-miR-342-3p | 350.1 | 1 331.1 | 3.80 | 3.43 | Y |
hsa-miR-182 | 316.1 | 1 653.1 | 5.23 | 11.86 | Y |
hsa-miR-200b | 1 362.1 | 4 162.1 | 3.06 | 12.61 | Y |
hsa-miR-183 | 118.1 | 1 067.1 | 9.04 | 6.12 | Y |
hsa-miR-423-3p | 2 446.1 | 1 993.1 | 0.81 | 5.96 | N |