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Table 2 Mutant Library Generated for ALLA_ECOLI and HCHA_ECOLI proteins of E.coli. The table shows a list of possible double mutants for wild type proteins and corresponding GRAVY values

From: In silico engineering of aggregation-prone recombinant proteins for substrate recognition by the chaperonin GroEL

S.No. Swissprot ID's Suggested Patches for Mutation (similar to GGIVLTG) Patches after mutation GRAVY value of mutated patch*
(Compare with that of GroES mobile loop)**
1 ALLA_ECOLI GDVIETQ GI VII TQ 1.871
  ALLA_ECOLI GDVIETQ GI VIL TQ 1.771
  ALLA_ECOLI GDVIETQ GI VIV TQ 1.828
  ALLA_ECOLI GDVIETQ GL VII TQ 1.771
  ALLA_ECOLI GDVIETQ GL VIL TQ 1.671
  ALLA_ECOLI GDVIETQ GL VIV TQ 1.728
  ALLA_ECOLI GDVIETQ GV VII TQ 1.828
  ALLA_ECOLI GDVIETQ GV VIL TQ 1.728
  ALLA_ECOLI GDVIETQ GV VIV TQ 1.785
2 HCHA_ECOLI GKLFSTG GI LFI TG 2.014
  HCHA_ECOLI GKLFSTG GI LFL TG 1.914
  HCHA_ECOLI GKLFSTG GI LFV TG 1.971
  HCHA_ECOLI GKLFSTG GL LFI TG 1.914
  HCHA_ECOLI GKLFSTG GL LFL TG 1.814
  HCHA_ECOLI GKLFSTG GL LFV TG 1.871
  HCHA_ECOLI GKLFSTG GV LFI TG 1.971
  HCHA_ECOLI GKLFSTG GV LFL TG 1.871
  HCHA_ECOLI GKLFSTG GV LFV TG 1.928
  1. As the single mutations for the patch doesn't make much difference in GRAVY value, so double mutations were considered.
  2. *The GRAVY values are calculated by using ProtParam tool (ExPasy)
  3. **The GRAVY value for mobile loop is = 1.514