Table 3 Pathogenicity and fitness factors identified from analysis of the genome of M. morganii KT
From: Whole-genome sequencing and identification of Morganella morganii KT pathogenicity-related genes
Category | Niche genes of M. morganii KT |
|---|---|
Drug resistance | Ampicillin resistance, ampC-ampR. Metallo-β-lactamases (MBL) MM2254, MM2308, and MM2606. Tellurite resistance operon MM933-1004. Tetracycline resistant, chloramphenicol, acetyltransferase, bicyclomycin, acetyltransferase, kasugamycin tetAJ, catA2, bcr, catB3-like, ksgA. |
| Â | Multidrug resistance and efflux genes (34 genes, Additional File 3: Supplementary table 2). |
Fimbrial adhesins | 3 MR/P operons, 13 mrpJ paralogous, 1 fimbrial chaperone, 2 UCA (NAF) operons, 1 PMF operon, and 2 other operons (Tables 4, 5). |
| Â | 6 putative type IV pili genes hofCB, ppdABCD, and 2 putative trimeric autotransporter secretion genes MM2011, MM2042. |
Motility/flagellum-related | Most flagellum-related genes and chemotaxis genes; MM1735-1785, MM1796-1797, and MM1786-1793 (at a single locus) (Additional File 4: Supplementary table 3). |
| Â | Methyl-accepting chemotaxis gene MM0264, aerotaxis gene MM1607, and 9 regulators of flagellar genes umoABCD, rssBA, rcsBCD. |
T3SS | Type III secretion system needle complex MM0224-MM0243 (20 genes), and effectors, ipaCBD-chaperone (MM0244-MM0247, 4 genes) (Additional File 5: Supplementary table 4) |
TCS | 19 potential two-component regulator systems (TCS) were identified. |
Iron acquisition system | Heme uptake, ferric, ferrous iron transport, ferric siderophore receptor, B12 transporter, and siderophore iron uptake hmuSTUV, afuABC, feoAB, ireA, btuCD, btuB, and yfeDCBA |
| Â | 18 other related genes (fecR, ABC transporters, TonB-dep. receptors) |
IgA protease | Zinc metalloprotease, capable of cleaving host Ig, zapABCD (MM1054-MM1056, MM1058) |
LPS, ECA & capsule | (1) Release lipid A, induce initial endotoxic shock and enterobacterial common antigen (41 genes, Additional File 6: Supplementary table 5) (2) Protect from phagocytosis, rcsB,C,D,F |
Haemolysins | hmpBA (MM2452, MM2453) |
Ureases | Urea as a nitrogen source, rapid urea hydrolysis, a cause of stone formation. ureABCEFGD-urea transporter (MM1961-MM1968) |
Insecticidal& apoptotic toxins | 9 insecticidal toxin: (1) tccB, tccA, and tcdB 2 (MM0965-MM0967), (2) xptA1A1C1 (MM1780-1782), (3) tcaC, tccB 3, and tcaC (MM1901, MM2567 and MM2570) (Additional File 7: Supplementary table 6) |
| Â | RtxA, XaxAB, intimin/invasion, HlyD toxin secretion, toxin transporter |
Other genes required for persistence of infection and fitness for infection | 6 superoxide stress genes katA, soxS, sodC,B,A, oxyR (Additional File 8: Supplementary table 7) The phage-shock-protein, pspABC operon (MM0627-MM0629) |
Ethanolamine utilisation | Ethanolamine utilisation system, provide carbon and/or nitrogen uptake; eutSPQTDMNEJGHABCLK-pduST-eutR (MM1148-MM1130). (Table 6) |
Cobalamin biosynthesis | Cobalamin (vitamin B12) biosynthesis, cbi-cob operon MM1167-MM1187. |