Table 2 Proportion of each serotype for pooling four serotypes of dengue templates and the calculated % detection of each pooled sample in both data sets
From: Feasibility of using 454 pyrosequencing for studying quasispecies of the whole dengue viral genome
Source | Strain/Sort | Mix ratio of DEN2 RNA. (%) | Mix ratio of four serotypes DENV purified DNA. (%) | Result from GS-FLX (%) | |
|---|---|---|---|---|---|
Data 1 | Data 2 | ||||
DENV1 | Hawaii | - | 18 | 18.72 | 16.55 |
DENV2 | 16681_mu1* | 29.9 | 46 | 32.62 | 19.89 |
16681 | 10 | 12.48 | 10.25 | ||
NGC | 5 | 8.14 | 6.06 | ||
16681-mu2* | 1 | 0.71 | 0.63 | ||
16681-mu3* | 0.1 | 0.00 | 0.00 | ||
DENV3 | H87 | - | 18 | 20.16 | 21.65 |
DENV4 | H241 | - | 18 | 7.17 | 24.96 |
Total | 46 | 100 | 100 | 100 | |