Figure 7

Donor variability for single genes. (A) Gene expression analysis by real-time RT-qPCR. COL2A1, SOX8 and SOX9 are shown relative to GAPDH expression for the individual donors. Note log10 scale. Each sample was measured in triplicate. Mean values shown, error bars reflect the Standard deviation. (B) Changes in histone modifications for individual donors. For H3K4me3, H3K27me3 and H3K9ac the sum of aligned reads within a 4 kb window around the TSS of all Ensembl transcripts of indicated genes is shown in both differentiation stages, while for H3K36me3 the sum of the reads for the longest transcript for each ensembl gene is given. (C) ChIP-seq signal tracks for donor 1 and 2 showing the indicated histone modifications in a 60 kb window around the TSS of indicated genes