Figure 4

Mutating DPEP reduces gene expression. The promoter of PITG_10185 (black bars), or a version with a mutated DPEP (white bars), were fused to the GUS reporter gene and transformed into P. infestans. Stable transformants were selected using the nptII marker and assayed for GUS activity using a fluorometric assay. Also shown is an alignment of the region of the PITG_10185 promoter that contains DPEP with orthologous promoters from P. capsici, P. ramorum, and P. sojae (PCAP, PRAM, and PSOJ, respectively). Numbers to the right of the alignment represent distances upstream of the start codon.