Figure 3

Summary of miRNA-target gene validations. In HEK293T-cells we tested the effects of a total of six miRNA-mimics (miR-10a, -22, -26a, 26b, 29b, and -137) on the 3^′-UTRs of 8 predicted proteins (CDK6, CTNNBIP1, DUSP2, HDAC4, SMAD1, SMAD6, TGFB3, and TOB1) related to osteogenic differentiation and/or function. Percent reductions of normalized firefly-activities from pmirGLO/3^′-UTR/miRNA-mimic co-transfections compared to pmirGLO/miRNA-mimic co-transfections are given in the colored squares. Red squares: lacking or non-significant miRNA effects, orange: weak but significant (p ≤0,05) miRNA effects, light green: moderate microRNA effects, dark green: strong microRNA effects. Among 22 individual miRNA-3^′-UTR interactions analyzed, 10 were counted as positive (light and dark green squares). *: predictions already validated in [32], **: predictions already validated in [31]. These were analyzed again here for reasons of comparability to our other validations. Detailed data from all experimental validations are listed in Additional file 2.