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Figure 5 | BMC Genomics

Figure 5

From: Systematic analysis of palatal transcriptome to identify cleft palate genes within TGFβ3-knockout mice alleles: RNA-Seq analysis of TGFβ3 Mice

Figure 5

Downstream effects analysis of CP gene patterns using IPA. The effects of TGFβ3 alleles on CP genes during palatogenesis were examined in the context of differentially expressed human and mouse genes using Ingenuity Pathway Analysis (IPA). Entrez IDs of each CP gene pattern/genotype group (n = 22) were uploaded to IPA as individual datasets. Each identifier was mapped to its corresponding gene object in the Ingenuity Knowledge Base (IKB). Interactions were then queried between these datasets and all other gene objects stored within IPA to generate a set of overlapping direct interaction networks. The significant genes were categorized, compared to functional categories in the IPA database, and ranked according to their p-values. The cellular processes that are most significantly altered in our datasets were: gene expression, cellular development, cellular growth and proliferation, cell signaling, cell morphology, cell death and survival, cellular movement, and cell cycle. The pattern-oriented distribution of the top cellular biofunctions were ranked and illustrated in a color coded matrix: the darker color represents greater relevance between cellular function and CP gene datasets.

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