Figure 1

Cell growth and differentiation of erythroid cells through ontogeny. (A) During erythropoiesis, the cell growth capacity was compared with number of seeded cells in the beginning of culture as fold induction of cell growth on a logarithmic scale. *P < 0.05 FL- versus mPB- and **P < 0.01 FL-derived erythroid cells versus all other cells. (B) Flow cytometry of erythroid progenitor cells, sorted as 100% CD71⁺ cells (gated on live CD71⁺ cells), is determined at day 6 of erythroid cultures. Values are mean ± SEM (n=2-4). (C) Histograms of CD34, CD36 and GLYA antigen expression on CD71⁺ sorted cells in a representative experiment at day 6 of erythroid cell culture. Values are positive cells for each marker expressed in percentage (%) of maximal number of cells.